Investigating the characterization of Hela CaMKK2 isoform mutations
Calcium/calmodulin-dependent protein kinase kinase 2 (CaMKK2) is a kinase enzyme involved in regulating many important physiological and pathophysiological processes. It is comprised in several different well-orchestrated series of sequences that help maintain homeostasis and alleviate ER stress in the body. Increased activity of this kinase is shown to be associated with cancer related diseases and regulated by mTOR, producing protein differences in CaMKK2 short and long isoforms. In this study, cell viability of Hela PV WT and CaMKK2 K/o cells were analyzed in response to a variety of functional assays to determine differences in cell susceptibility and changes in gene expression. To do this, cells of each type were grown in culture plates, treated with respective medias and analyzed through western blotting. Results displayed that Hela PV WT cells showed higher cell population and faster doubling time than knockout cells. Further analysis suggests that Hela PV WT and CaMKK2 K/o show differences in phosphorylation activity and response to downstream events. However, characteristics between each isoform were not conclusively determined to show a significant difference.