Durability Study of LamLG3 and Net1 Peptides Coated Glass Surfaces Prepared Via Silanization and Copper-free Click Chemistry with Dibenzocyclooctyne-amine
Various lab studies have shown that different surface coatings on medical devices are able to provide numerous beneficial functions. Upon scale-up and distribution to clinics, medical devices’ long manufacturing and shipping time and their encounters with all kinds of challenges during clinical applications become critical. Device coatings’ ability to retain their functions and remain effective over a long period is significant for sustainable performance, reduction of manufacturing waste, and reduced patient cost. Therefore, durability tests of peptide-coated surfaces are necessary. Here, two cell adhesion peptides derived from basement membrane biomolecules---Net1 and LamLG3---were each coated onto glass disk surfaces via silanization and copper-free click chemistry with dibenzocyclooctyne-amine (DIBO). These two types of promising coating were excessively challenged by (1) immersion in artificial saliva for up to 8 weeks, (2) autoclaving for up to 5 times, and (3) toothbrushing for up to 68 minutes. It was found that 2 weeks in the saliva, 1 time of autoclaving, and 1.2 minutes of toothbrushing all notably degraded the two peptide coating layers on glass surfaces. These findings may catalyze the creation of a generic model of the peptide coatings’ resistance against various challenges involved in medical device manufacture and post-implantation for improvements of related surface coating designs and clinical applications.