Transcriptomic Data Analysis of Up regulated Transporter Genes in Nectary Cells of Arabidopsis thaliana
Overall, the goal of this research was to uncover trends in the transcriptomic data that elucidated which genes were of importance to the nectary cell tissue. More specifically, we wanted to understand the importance of these genes in the Mature Lateral Nectary (MLN). This goal was achieved by filtering and sorting Xcel files, which contained values with arbitrary units that represented expression levels. Data was available for the expression in tissue types such as the leaf, root, nectaries, stem, as well as others tissue types. Through this filtering, 12 transport related genes were found that showed greater than five-fold increases in expression in mature lateral nectary cells over other common reference tissue such as mature petal tissue, mature root tissue, and rosette leaf tissue. Following the filtering of these genes, extensive research into the nature of these transporters was conducted such as discovering the number of transmembranous regions, as well as protein localization, in order to confirm the nature of their function as well as elucidate the specifics of each gene.