Evaluation of the Activities of N1-biphenyl Quinazolinediones
Quinazolinediones are structurally similar to fluoroquinolones and inhibit topoisomerases. Fluoroquinolones compose the largest class of antibiotics on the market due to their ability to inhibit and poison bacterial topoisomerases, but in eukaryotic models, topoisomerase poisoning creates secondary malignancies. Recently, an addition of a biphenyl group at the N-1 position of several fluoroquinolones showed inhibition of type I eukaryotic topoisomerases without poisoning effects. Though structurally similar, it is unknown if adding an N-1 biphenyl group to quinazolinediones would also be successful in stopping topoisomerase poisoning and the formation of secondary malignancies. The activity of N-1 biphenyl quinazolinediones was assessed using a relaxation assay and a MTT assay and MTT assays. Activities of the tested quinazolinedione were found to be similar to the active flouroquinolones. This research can be further studied to implement quinazolinediones as a future anticancer drug.