Sublethal dose of irradiation disrupts migration and cell cycle dynamics of glioma cells
Glioblastoma Multiforme (GBM) is a lethal malignant brain tumor due to its invasive and migratory features. Current treatment options include a multimodal therapeutic approach of neurosurgery, standard radiotherapy, and concomitant radiotherapy and chemotherapy. However, the migration and invasiveness of glioma cells post-irradiation are still not well understood and are debatable across studies. In this study, we applied a 2.5-dimension migration assay to irradiated glioma cells at the sublethal dosage of 10 Gy on collagen type I hydrogels. We found that irradiated glioma cells significantly and continuously reduced their migration speed and proliferation with increased cell death in 8 days, while control cells had consistent migration speed and proliferation. These results demonstrate that a strong dose of radiation can significantly disrupt the dynamics of the cell cycle and cell migration. Future experiments will be conducted to better understand the invasiveness and resistance of glioma cells post-irradiation with varying radiation doses and microenvironments.