Characterization of ETV2-Hsp68-EYFP Mouse Embryos
ETV2, or ER71, is a pioneer transcription factor that regulates chromatin remodeling and transcription rate. Previous studies have shown that ETV2 has an essential role in mesodermal development, but further studies are needed to thoroughly investigate this gene's function. In this study, the University of Texas Southwestern transgenic core facility produced a transgenic line (ETV2-Hsp68-EYFP) in which a 3.9 Kb ETV2 promoter is fused with an Hsp68 minimal promoter and an EYFP reporter sequence. Positive ETV2-Hsp68-EYFP males were bred with wild-type CD1 and B6 female mice. Characterization of embryos at various embryonic stages was done to understand the role of ETV2 in early embryonic development. Different scientific techniques, such as FACS and immunohistochemistry, were performed on the positive EYFP cell populations. Further analyses need to be performed to define the role of ETV2 in the development of hematopoietic and endothelial lineages.